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KMID : 0624620110440110753
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BMB Reports
2011 Volume.44 No. 11 p.753 ~ p.757
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Dimethylsulfoxide (DMSO) induces downregulation of heme oxygenase-1 (HO-1) in HL-60 cells: involvement of HO-1 in HL-60 cell differentiation
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Noh Eun-Mi
Cho Dong-Hyu
Lee Young-Rae
Jeong Young-Ju
Kim Jong-Hyeon
Chae Hee-Suk
Park Jinny
Jung Won-Seok
Park Sung-Joo
Kim Jong-Suk
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Abstract
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Heme oxygenase-1 (HO-1), an inducible enzyme with broad tissue expression, is wel1-regulated in response to hematopoietic stress and preserves vascular homeostasis. We investigated the involvement of HO-1 in HL-60 cell differentiation. Dimethyl sulfoxide (DMSO) completely decreased HO-1 expression in a time-dependent manner, but clearly induced HL-60 cell differentiation, as evidenced by a marked increase in CD11b expression. Interestingly, zinc protoporphyrin (ZnPP), a strong inhibitor of HO-1, induced HL-60 cell differentiation. In contrast, treatment with cobalt protoporphyrin (CoPP), an activator of HO-1, decreased CD11b expression. Additionally, ZnPP downregulated HO-1 protein expression in HL-60 cells, whereas CoPP induced upregulation. These results suggest that HO-1 might have a negative function in DMSO-induced HL-60 cell differentiation. This study provides the first evidence that HO-1 plays an important role in DMSO-induced HL-60 cell differentiation.
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KEYWORD
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Differentiation, DMSO, Heme oxygenase-1, HL-60 cells, Protoporphyrin
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